Resultado de búsqueda
24 de ago. de 2017 · We sought to determine the binding affinities of the 10 β-lactam antibiotics for PBP1, PBP2, PBP3, and PBP4 of S. aureus using a competition fluorescence anisotropy assay and Bocillin (Thermo Fisher Scientific, Waltham, MA) as the fluorescent ligand.
22 de jul. de 2022 · S. aureus strains have evolved resistance mechanisms to almost all antimicrobial drugs used in treatment. The most important is resistance to drugs most commonly used in the treatment of Gram-positive infections, i.e., beta-lactams, glycopeptides, and oxazolidinones.
15 de jun. de 2022 · S. aureus DivIB is a PG-binding protein essential for division, the depletion of which leads to septal plate formation loss (34, 35). Here, the PBP1 PASTA domains were found to be involved in binding to DivIB and FtsW, alluding to their essential role in cell division.
22 de jun. de 2018 · Effectiveness of β-lactams is severely compromised due to resistance, which is widespread among clinical strains of Staphylococcus aureus. β-lactams inhibit bacterial cells by binding to penicillin binding proteins (PBPs), which perform the penultimate steps of bacterial cell wall synthesis.
In vitro data on purified recombinant S. aureus PBP4 suggest that it is a beta-lactamase and is not trapped as an acyl intermediate with beta-lactam antibiotics. Put together, the expression analysis and biochemical features of PBP4 provide a framework for understanding the function of this protein in S. aureus and its role in antimicrobial ...
PBP2a confers resistance to β-lactam antibiotics in Staphylococcus aureus. We expressed the gene encoding PBP2a in a β-lactam sensitive strain of S. aureus. We found that the resistance level conferred by PBP2a varies considerably between β-lactams and between single cells in a population.
25 de nov. de 2004 · Recruitment of PBP2 to the cell division site is prevented after acylation by oxacillin. To determine the effect of acylation by β-lactam antibiotics, strain RNpPBP2-31 was grown to early mid-exponential phase in tryptic soy broth (TSB) and treated with oxacillin (4 µg ml −1 ).
