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  1. 24 de feb. de 2023 · The RNA-sequencing data analysis pipeline can be divided into three major parts: data pre-processing, followed by the main and downstream analyses. Here, we present an overview of the tools used in both the bulk RNA-seq and at the single-cell level, with a particular focus on alternative splicing and active RNA synthesis analysis.

    • PubMed

      The RNA-sequencing data analysis pipeline can be divided...

  2. 24 de jul. de 2019 · RNA sequencing (RNA-seq) was developed more than a decade ago 1, 2 and since then has become a ubiquitous tool in molecular biology that is shaping nearly every aspect of our understanding of...

  3. 24 de feb. de 2023 · The RNA-sequencing data analysis pipeline can be divided into three major parts: data pre-processing, followed by the main and downstream analyses. Here, we present an overview of the tools used in both the bulk RNA-seq and at the single-cell level, with a particular focus on alternative splicing and active RNA synthesis analysis.

  4. 1 de ene. de 2020 · In this review we provide a comprehensive historical background of the improvements in DNA sequencing technologies that have accompanied the major milestones in genome sequencing and assembly, ranging from early sequencing methods to Next-Generation Sequencing platforms.

  5. A series of milestone publications reported the development of high-throughput sequencing of whole transcriptomes, known as RNA sequencing (RNA-seq), across different species. Read more.

  6. 14 de sept. de 2021 · The RNA sequence used in the COVID-19 vaccine developed by Pfizer and BioNTech (Ψ is a modified form of the uridine nucleotide, U). Credit: Nik Spencer/Nature. In late 1987, Robert Malone...

  7. 4 de dic. de 2020 · This review summarizes the history of the development of RNA sequencing and focuses on the latest studies of RNA sequencing technology in cancer applications, especially single-cell RNA sequencing and spatial transcriptome sequencing.