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29 de mar. de 2024 · Through structure-guided engineering of human APOBEC3A (A3A) deaminase, we developed highly accurate A3A-CBE (haA3A-CBE) variants that efficiently generate C-to-T conversion with a narrow editing...
Here we describe a strategy for reducing bystander mutations using an engineered human APOBEC3A (eA3A) domain, which preferentially deaminates cytidines in specific motifs according to a TCR>TCY>VCN hierarchy.
30 de jul. de 2018 · Here we describe a strategy for reducing bystander mutations using an engineered human APOBEC3A (eA3A) domain, which preferentially deaminates cytidines in specific motifs according to a...
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27 de nov. de 2021 · Here we describe a strategy for reducing bystander mutations using an engineered human APOBEC3A (eA3A) domain, which preferentially deaminates cytidines in specific motifs according to a...
Purpose. CAG promoter expression plasmid for hA3A (N57G)-XTEN linker-hSpCas9n (D10A)-UGI-NLS-P2A-EGFP. Depositing Lab. Keith Joung. Publication. Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038/s41587-019-0236-6. doi: 10.1038/s41587-019-0236-6. ( How to cite ) Sequence Information. Sequences (2) Enlarge. View all sequences. Ordering.